Part:BBa_K3071023:Design
Co-activator construct for activating synthetic clp-regulated pspA promoter with sequencing part
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 953
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 956
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 953
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 953
Illegal NgoMIV site found at 1031
Illegal AgeI site found at 136
Illegal AgeI site found at 164
Illegal AgeI site found at 302
Illegal AgeI site found at 2509 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Condon optimization for E. coli expression; Two sequencing primers flanked the whole construct for clone screening
Source
-
References
1. Chen, C. H., Lin, N. T., Hsiao, Y. M., Yang, C. Y., & Tseng, Y. H. (2010). Two non-consensus Clp binding sites are involved in upregulation of the gum operon involved in xanthan polysaccharide synthesis in Xanthomonas campestris pv. campestris. Research in microbiology, 161(7), 583-589.
2. Dworkin, J., Jovanovic, G., & Model, P. (1997). Role of upstream activation sequences and integration host factor in transcriptional activation by the constitutively active prokaryotic enhancer-binding protein PspF. Journal of molecular biology, 273(2), 377-388.
3. Rappas, M., Schumacher, J., Beuron, F., Niwa, H., Bordes, P., Wigneshweraraj, S., ... & Zhang, X. (2005). Structural insights into the activity of enhancer-binding proteins. Science, 307(5717), 1972-1975.
4. Ryan, R. P., & Dow, J. M. (2011). Communication with a growing family: diffusible signal factor (DSF) signaling in bacteria. Trends in microbiology, 19(3), 145-152.
5. Transcription Initiation by σ54. (n.d.). Retrieved from http://www.cchem.berkeley.edu/wemmer/research/sigma54.html.
6. Wigneshweraraj, S., Bose, D., Burrows, P. C., Joly, N., Schumacher, J., Rappas, M., ... & Buck, M. (2008). Modus operandi of the bacterial RNA polymerase containing the σ54 promoter‐specificity factor. Molecular microbiology, 68(3), 538-546.
7. Zhou, Y., Asahara, H., Schneider, N., Dranchak, P., Inglese, J., & Chong, S. (2014). Engineering bacterial transcription regulation to create a synthetic in vitro two-hybrid system for protein interaction assays. Journal of the American Chemical Society, 136(40), 14031-14038.
8. Transcription Initiation by σ54. (n.d.). Retrieved from http://www.cchem.berkeley.edu/wemmer/research/sigma54.html.